Investigation of Hyperglycemia-specific RNAs in Type 2 Diabetes

Investigation of hyperglycemia specific circulating non-coding RNAs in type 2-diabetes

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The project is underway. For more info, please send an email at queries@shf.org.pk

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 Dr.  Muhammad Tariq  (LUMS)  and Dr. Rozina Arshad  (SiDER) 

 

The overarching goal of this study is to identify circulating non-coding RNA from blood samples in type 2-diabetes (T2DM) and correlate these non-coding RNA molecules with hyperglycemia mediated epigenetic changes in patient genome.

Hyperglycemia results in epigenetic changes which involve chromatin modifications and non-coding RNAs like microRNAs and long non-coding RNA (lncRNA) having an effect on regulation of genes like pro-inflammatory cytokines and development of comorbid conditions like cardiovascular disease in diabetes.

The hypothesis will be tested using the following aims

  1. Measure the effect of hyperglycemia on global gene regulation through RNAseq analysis and identify diabetes specific gene expression profile
  2. Identify non-coding RNAs, in particular micro RNAs and correlate their presence with hyperglycemia
  • Determine diabetes specific role of non-coding RNA and their effect on levels of pro-inflammatory cytokines (IL-6, TNF-alpha, and IL-1) in individuals with type 2-diabetes (T2DM)