The overarching goal of this study is to identify circulating non-coding RNA from blood samples in type 2-diabetes (T2DM) and correlate these non-coding RNA molecules with hyperglycemia mediated epigenetic changes in patient genome.
Hyperglycemia results in epigenetic changes which involve chromatin modifications and non-coding RNAs like microRNAs and long non-coding RNA (lncRNA) having an effect on regulation of genes like pro-inflammatory cytokines and development of comorbid conditions like cardiovascular disease in diabetes.
The hypothesis will be tested using the following aims
- Measure the effect of hyperglycemia on global gene regulation through RNAseq analysis and identify diabetes specific gene expression profile
- Identify non-coding RNAs, in particular micro RNAs and correlate their presence with hyperglycemia
- Determine diabetes specific role of non-coding RNA and their effect on levels of pro-inflammatory cytokines (IL-6, TNF-alpha, and IL-1) in individuals with type 2-diabetes (T2DM)